ELISA Vascular Endothelial cell Growth Factor B (VEGF-B)

Reactivity: (Homo sapiens) UniProt:P49765 Abbreviation:VEGFB Alternative Names:VEGFL; VRF; Application:ELISA Range:15.6-1000 pg/mL Sensitivity:5.9 pg/mL Intra-AssayCV:?4.8% Inter-AssayCV:?8.1% Recovery:1.02 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate VEGFB in samples. An antibody specific for VEGFB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVEGFB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for VEGFB is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VEGFB bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:VascµLar endothelial growth factor B is a growth factor that belongs to the vascµLar endothelial growth factor family of which VEGF-A is the best known member. In contrast to VEGF-A, VEGF-B plays a less pronounced role in the vascµLar system: whereas VEGF-A is important for the formation of blood vessels, such as during development or in pathological conditions, VEGF-B seems only to play in role in the maintenance of newly formed blood vessels during pathological conditions. VEGF-B plays also an important role on several types of neurons. It is important for the protection of neurons in the retina and the cerebral cortex during stroke and of motoneurons during motor neuron diseases such as amyotrophic lateral sclerosis.VEGF-B exerts its effects via the FLT1 receptor. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).