ELISA Vitamin B1 (VB1)

Reactivity: (Homo sapiens) UniProt:N/A Abbreviation:VB1 Alternative Names:N/A Application:ELISA Range:3.70-300 ng/mL Sensitivity:1.63 ng/mL Intra-AssayCV:?6.0% Inter-AssayCV:?11.3% Recovery:1.03 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate VB1 in samples. An antibody specific for VB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for VB1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VB1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Thiamine or thiamin, sometimes called aneurin, is a water-soluble vitamin of the B complex (vitamin B1), whose phosphate derivatives are involved in many cellµLar processes. The best characterized form is thiamine diphosphate (ThDP), a coenzyme in the catabolism of sµgars and amino acids. In yeast, ThDP is also required in the first step of alcoholic fermentation. Thiamine is syntheQuantityd in bacteria, fungi and plants. Animals must cover all their needs from their food and insufficient intake resµLts in a disease called beriberi affecting the peripheral nervous system (polyneuritis) and/or the cardiovascµLar system, with fatal outcome if not cured by thiamine administration. In less severe deficiency, nonspecific signs include malaise, weight loss, irritability and confusion. Today, there is still a lot of work devoted to elucidating the exact mechanisms by which thiamine deficiency leads to the specific symptoms observed Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).