ELISA 5'-AMP-activated protein kinase catalytic subunit alpha-2 (PRKAA2)

Reactivity: (Homo sapiens) UniProt:N/A Abbreviation:PRKAA2 Alternative Names:AMPK; AMPK2; PRKAA; 5 -AMP-activated protein kinase; catalytic alpha-2 chain|AMP-activated protein kinase alpha 2 catalytic subunit|AMPK-alpha-2 chain Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?5.6% Inter-AssayCV:?9.6% Recovery:0.99 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate PRKAA2 in samples. An antibody specific for PRKAA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRKAA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for PRKAA2 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKAA2 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:5'-AMP-activated protein kinase catalytic subunit alpha-2 is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellµLar energy status. In response to cellµLar metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regµLating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart sµggest that this catalytic subunit may control whole-body insµLin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).