ELISA Vesicular integral-membrane protein VIP36 (LMAN2)
Reactivity: (Homo sapiens)
UniProt:Q12907
Abbreviation:LMAN2
Alternative Names:C5orf8; GP36B; VIP36; vesicµLar integral protein of 36 kDa
Application:ELISA
Range:0.156-10 ng/mL
Sensitivity:0.055 ng/mL
Intra-AssayCV:?6.9%
Inter-AssayCV:?8.9%
Recovery:0.92
Sample Type:Serum, Plasma, Other biological fluids
Detection Method:Sandwich
Analysis Method??:Quantitive
Test principle:This assay employs a two-site sandwich ELISA to quantitate LMAN2 in samples. An antibody specific for LMAN2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLMAN2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for LMAN2 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LMAN2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:By searching an EST database for sequences similar to dog Vip36, Neve et al. (2003) identified LMAN2, which they called VIP36. The deduced 356-amino acid protein contains an N-terminal signal sequence, followed by a lectin-type carbohydrate recognition domain and a C-terminal transmembrane domain. A KRFY endoplasmic reticµLum trafficking motif is located at the C terminus. Northern blot analysis detected variable expression of 1.2- and 1.4-kb VIP36 transcripts in all tissues examined. Highest expression was in kidney, placenta, and liver. Nufer et al. (2003) determined that the LMAN2 gene contains 8 exons and spans 14.9 kb.By genomic sequence analysis, Nufer et al. (2003) mapped the LMAN2 gene to chromosome 5q35.5.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).