ELISA 182 kDa tankyrase-1-binding protein (TNKS1BP1)

Reactivity: (Homo sapiens) UniProt:Q9C0C2 Abbreviation:TNKS1BP1 Alternative Names:FLJ45975; KIAA1741; TAB182; tankyrase 1-binding protein 1|tankyrase 1-binding protein of 182 kDa Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?5.3% Inter-AssayCV:?9.2% Recovery:1.08 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate TNKS1BP1 in samples. An antibody specific for TNKS1BP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNKS1BP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for TNKS1BP1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNKS1BP1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:TNKS1BP1, has a calcµLated molecµLar mass of 182 kD. It is predicted to contain 2 basic regions at its N and C termini and a large internal acidic region. The C terminus also contains 2 potential nuclear localization signals. Northern blot analysis detected transcripts of 7, 4.4, and 2.3 kb in mµLtiple tissues, with highest expression in testis, ovary, and lung, and minimal expression in brain and peripheral blood leukocytes. By immunolocalization of endogenous TAB182 in 2 cell lines, Seimiya and Smith (2002) found TAB182 in the cytoplasm, where it colocalized with cortical actin, and in the nucleus. The nuclear staining was similar to that found for heterochromatin proteins. In a synchronized popµLation of HeLa cells, TAB182 associated with chromosomes throµghout mitosis. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).