ELISA Vascular endothelial cell growth factor (VEGF165)

Reactivity: (Homo sapiens) UniProt:N/A Abbreviation:VEGF165 Alternative Names:N/A Application:ELISA Range:15.6-1000 pg/mL Sensitivity:5.8 pg/mL Intra-AssayCV:?4.9% Inter-AssayCV:?9.4% Recovery:1.07 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate VEGF165 in samples. An antibody specific for VEGF165 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVEGF165 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for VEGF165 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VEGF165 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:VEGF-A is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascµLar permeability, inducing angiogenesis, vascµLogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. VEGF is a chemical signal produced by cells that stimµLates the growth of new blood vessels. The most important member is VEGF-A. Other members are Placenta growth factor (PlGF), VEGF-B, VEGF-C and VEGF-D. The latter ones were discovered later than VEGF-A, and, before their discovery, VEGF-A was called just VEGF. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).