ELISA Vascular Endothelial cell Growth Factor C (VEGF-C)

Quantity:96T. Other Quantitys are also available. Please Inquire. Reactivity: (Homo sapiens) UniProt:P49767 Abbreviation:VEGFC Alternative Names:Flt4-L; VRP; FLT4 ligand DHM|vascµLar endothelial growth factor-related protein Application:ELISA Range:15.6-1000 pg/mL Sensitivity:5.8 pg/mL Intra-AssayCV:≤4.3% Inter-AssayCV:≤8.2% Recovery:1,04 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate VEGFC in samples. An antibody specific for VEGFC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVEGFC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for VEGFC is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VEGFC bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:VEGF-C has been shown to exhibit angiogenic and lymphangiogenic actions. The VEGF family of growth factors and receptors is involved in the development and growth of the vascµLar endothelial system. Two of its family members, VEGF-C and VEGF-D, regµLate the lymphatic endothelial cells via their receptor VEGFR, thus acting as mitogens for these cells. VEGF-C expression is associated with hematological malignancies. Like VEGF it acts as survival factor on leukemia. Together with the expression of their receptors, VEGF and VEGF-C resµLt in the generation of autocrine loops that may support cancer cell survival and proliferation. Further VEGF-C expression has been shown in gastrointestinal tract malignancies where it correlates with lymphatic invasion, lymphnode metastasis and reduced survival. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).