ELISA WNT1-inducible-signaling pathway protein 1 (WISP1)

Reactivity: (Homo sapiens) UniProt:O95388 Abbreviation:WISP1 Alternative Names:CCN4; WISP1c; WISP1i; WISP1tc; WNT1 induced secreted protein 1|Wnt-1 inducible signaling pathway protein 1|wnt-1 signaling pathway protein 1 Application:ELISA Range:31.25-2000 pg/mL Sensitivity:12.1 pg/mL Intra-AssayCV:?5.3% Inter-AssayCV:?10.2% Recovery:1.03 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate WISP1 in samples. An antibody specific for WISP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWISP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for WISP1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WISP1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:WISP1 encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellµLar matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage throµgh activation of the Akt kinase. It is 83% identical to the mouse protein at the amino acid level. Alternative splicing of this gene generates 2 transcript variants. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).